Life Science Companies

Featured
New Product

EMD Chemicals introduces the new Calbiochem® fully validated antibodies targeting areas of cancer, apoptosis, cardiovascular disease, and much more!

Featured Catalog

Braintree Scientific
Over 30 years servicing
the needs of the life science industry.
Order our free catalog now!!

Free Featured Articles at Cold Spring Harbor Protocols

Construction and Characterization of Adenovirus Vectors
Systematic Monitoring of Protein Complex Composition and Abundance by Blue-Native PAGE
Dye Loading with Patch Pipettes
Optimization and Troubleshooting in PCR
Virus-Induced Gene Silencing as a Tool for Delivery of dsRNA into Plants
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
Xenopus laevis Keller Explants
Whole-Mount In Situ Hybridization of RNA Probes to Plant Tissues
Using the Basic Local Alignment Search Tool (BLAST)
Transcript In Situ Hybridization of Whole-Mount Embryos for Phenotype Analysis of RNAi-Treated Drosophila
The Genus Antirrhinum (Snapdragon): A Flowering Plant Model for Evolution and Development
Studying Mitosis in Cultured Mammalian Cells
Staining Whole Mouse Embryos for ß-Galactosidase (lacZ) Activity
SNP Mining from Maize 454 EST Sequences
Selection of Appropriate Imaging Equipment and Methodology for Live Cell Imaging in Drosophila
Quantitation of DNA and RNA
Production of Endoribonuclease-Prepared Short Interfering RNAs (esiRNAs) for Specific and Effective Gene Silencing in Mammalian Cells
Preparing Recombinant Gonad Organ Cultures
Preparation of Rodent Hippocampal Slice Cultures
Preparation of GST Fusion Proteins
Preparation of Complex DNA Probe Sets for 3D FISH with up to Six Different Fluorochromes
Planarians: A Versatile and Powerful Model System for Molecular Studies of Regeneration, Adult Stem Cell Regulation, Aging, and Behavior
Photoactivation-Based Labeling and In Vivo Tracking of RNA Molecules in the Nucleus
Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
Mouse Mutagenesis Using N-Ethyl-N-Nitrosourea (ENU)
Microinjection of Protein Samples
Microinjection of dsRNA into Mouse Oocytes and Early Embryos
Methylated CpG Island Amplification and Microarray (MCAM) for High-Throughput Analysis of DNA Methylation
Measurement of Courtship Behavior in Drosophila melanogaster
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
Maintenance of a Drosophila Laboratory: General Procedure
Live Imaging of Caenorhabditis elegans: Examples
Live-Cell Imaging of GFP in Plants
Karyotyping Mouse Cells
Isolation, Culture, and Differentiaton of Progenitor Cells from the Central Nervous System
Isolation and Whole-Cell Patch Clamping of Arabidopsis Guard Cell Protoplasts
In Vivo Isotopic Labeling of Proteins for Quantitative Proteomics
In Vitro Sumoylation of Recombinant Proteins and Subsequent Purification for Use in Enzymatic Assays
In Vitro Screening for Regulated Transcription Factors with Differential Display of DNA-Binding Proteins (DDDP)
In Vitro Assays for the Extracellular Matrix Protein-Regulated Extravasation Process
In Planta Transformation of Arabidopsis
Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)
Immunohistochemistry of Whole-Mount Mouse Embryos
HPLC Separation of Digested Proteins and Preparation for Matrix-Assisted Laser Desorption/Ionization Analysis
Genotyping by Oligonucleotide Ligation Assay (OLA)
Gateway-Compatible Yeast One-Hybrid Screens
Fetal Thymus Organ Culture
Fate-Mapping Technique: Using Carbocyanine Dyes for Vital Labeling of Cells in Gastrula-Stage Mouse Embryos Cultured in Vitro
Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
DNA Immunoprecipitation (DIP) for the Determination of DNA-Binding Specificity
Development of Mammalian Cell Lines with lac Operator-Tagged Chromosomes
Design and Cloning of an shRNA into a Lentiviral Silencing Vector: Version A
Cutaneous Two-Stage Chemical Carcinogenesis
Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
Cultures of Cerebellar Granule Neurons
Copy Number Variation Detection via High-Density SNP Genotyping
Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content
Chromatin Immunopreciptation (ChIP) on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications
Chromatin Immunoprecipitation (ChIP) of Protein Complexes: Mapping of Genomic Targets of Nuclear Proteins in Cultured Cells
Choosing a Method for Phylogenetic Prediction
Browsing HapMap Data Using the Genome Browser
Bone Marrow-Derived Macrophages (BMM): Isolation and Applications
Assays with Protein Arrays
Assay for Human Rad51-Mediated DNA Displacement Loop Formation
A Sealed Preparation for Long-Term Observations of Cultured Cells
Analysis of Protein Interactions with Immobilized Peptide Arrays Synthesized on Membrane Supports
Analysis of Complex Protein Mixtures Using Multidimensional Protein Identification Technology (MuDPIT)