SUPPLIER DETAILS

Clontech Laboratories Inc

Clontech Laboratories, Inc., a wholly-owned subsidiary of Takara Bio Inc., develops, manufactures, and distributes a wide range of life science research reagents including high-performance qPCR and PCR reagents (including the Titanium™ and Advantage® enzymes); RT enzymes and SMART™ library construction kits; the innovative In-Fusion® cloning system; Tet-based inducible gene expression systems; and a range of Macherey-Nagel nucleic acid purification tools. These and other products support applications including gene discovery, regulation, and function; protein expression and purification; RNAi and stem cell studies; and plant and food research.

· Anti-DYKDDDDK antibody detects the DYKDDDDK (FLAG) tag; no cross-reactions with endogenous proteins.

· Plasmid midi prep kits to purify 500 µg transfection-grade DNA in 28 minutes.

· His tag protein purification with 60 mg/ml capacity and better purity than Ni-NTA resins.

· PCR clean up and gel extraction kit to purify DNA from agarose gels and PCR reactions.

· Xfect™ transfection reagent transfects plasmid DNA into many types of cells with low cytotoxicity and high transfection efficiency.

· siRNA transfection reagent for better gene silencing than the leading competitor.

· Protein transfection reagent transports active proteins directly into a wide variety of mammalian cells.

· Fetal bovine serum undergoes rigorous functional testing, for optimal tetracycline-inducible expression.

· LDH kit to measure cell death—detect as few as 2,000 dead cells/well.

· WST-1 kit to measure cell proliferation in 1 hour. More stable than other WST-1 reagents.

· Fluorescent protein antibodies to detect fluorescent proteins. Use for westerns, IP and IC.

· RT-PCR kit: RNA to cDNA EcoDry™ Premixes are lyophilized RT PCR master mixes. Store at room temperature.

· qPCR Premix: use a 2X master mix for real-time PCR contains SYBR® Green I

· Direct polymerase mix allows you to skip sample prep and amplify directly from animal or plant tissues.

· High-fidelity PCR premix: a lyophilized master mix yields long and accurate PCR products.